2H4I
Crystal structure of the complex of proteolytically produced C-terminal half of bovine lactoferrin with lactose at 2.55 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.441, 50.417, 65.890 |
| Unit cell angles | 90.00, 107.84, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b65 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.710 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 12469 | |
| <I/σ(I)> | 6.8 | 1.8 |
| Completeness [%] | 94.8 | 71.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M MES, 25% POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 0.01M ZINC SULPHATE , pH 6.5, VAPOR DIFFUSION, temperature 298K |
