2H3M

Crystal Structure of ZO-1 PDZ1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-06-20
Detector	MAR scanner 345 mm plate
Spacegroup name	H 3 2
Unit cell lengths	62.789, 62.789, 153.531
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.23
Rwork	0.227
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 2H3L
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge		0.034
Number of reflections	3037
<I/σ(I)>	34.2		2.6
Completeness [%]	99.8	99.7	98
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	292	0.1 M Sodium Acetate, 0.2 M Ammonium Sulfate, 30% PEG 2000 Monomethyl Ether, pH 4.6, vapor diffusion, temperature 292K
1	VAPOR DIFFUSION	4.6	292	0.1 M Sodium Acetate, 0.2 M Ammonium Sulfate, 30% PEG 2000 Monomethyl Ether, pH 4.6, vapor diffusion, temperature 292K