2H3L

Crystal Structure of ERBIN PDZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-02
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	38.864, 44.032, 57.265
Unit cell angles	90.00, 106.18, 90.00

Refinement procedure

Resolution	30.000 - 1.000
Rwork	0.148
R-free	0.16900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1MFG
RMSD bond length	0.011
RMSD bond angle	1.518
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.040
High resolution limit [Å]	1.000	1.000
Number of reflections	100042
<I/σ(I)>	19.5	1.6
Completeness [%]	99.4	97.6
Redundancy	3.2	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	292	0.1 M TRIS-HCL, 35% PEG 4000, pH 7.5-8.5, vapor diffusion, temperature 292K, pH 7.50
1		7.5	292	0.1 M TRIS-HCL, 35% PEG 4000, pH 7.5-8.5, vapor diffusion, temperature 292K, pH 7.50