2H3H
Crystal structure of the liganded form of Thermotoga maritima glucose binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0357 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.200, 46.060, 118.400 |
| Unit cell angles | 90.00, 108.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.20502 |
| Rwork | 0.203 |
| R-free | 0.24108 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dri |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.172 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 79951 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 95.1 | 88.9 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 17% PEG 3350, 0.2M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
