2H3D

Crystal Structure of Mouse Nicotinamide Phosphoribosyltransferase/Visfatin/Pre-B Cell Colony Enhancing Factor in Complex with Nicotinamide Mononuleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2005-07-03
Spacegroup name	P 1 21 1
Unit cell lengths	60.460, 107.960, 83.650
Unit cell angles	90.00, 96.98, 90.00

Refinement procedure

Resolution	40.130 - 2.100
R-factor	0.215
Rwork	0.215
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	61073
<I/σ(I)>	13.4	2.7
Completeness [%]	98.9	92.6
Redundancy	3.2	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8	291	5 mg/ml Se-Met Nampt with 10 mM added NMN and reservoir solution containing 0.1 M HEPES pH 7.8, 0.1 M Na acetate, and 14.5% PEG4000 in a 1:1 ratio in a total initial drop volume of 2 microliter., VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 7.80