2H32

Crystal structure of the pre-B cell receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-21
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	71.502, 71.502, 217.932
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.269
Rwork	0.267
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1IT9 AND 1ADQ
RMSD bond length	0.014
RMSD bond angle	1.272
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.850
High resolution limit [Å]	2.700	2.700
Number of reflections	16403
<I/σ(I)>	7	2
Completeness [%]	99.9	100
Redundancy	8.1	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	293	12% MP5K, 200mM Zinc acetate, 100mM PIPES, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 293K