2H2Y

Crystal structure of ubiquitin conjugating enzyme E2 from plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-03-29
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	66.117, 85.414, 93.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.800 - 2.800
R-factor	0.282
Rwork	0.232
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fo3
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	13521
<I/σ(I)>	38.4
Completeness [%]	100.0	100
Redundancy	7.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	291	29% Peg 3350, 0.1M (NH4)2SO4, 0.1M Bis-Tris, 5% Glycerol, pH 6.4, VAPOR DIFFUSION, temperature 291K