2H2J

Structure of Rubisco LSMT bound to Sinefungin and Monomethyllysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	95
Detector technology	CCD
Collection date	2004-08-13
Detector	MAR CCD 165 mm
Wavelength(s)	0.9686
Spacegroup name	I 2 2 2
Unit cell lengths	132.370, 156.500, 267.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.790 - 2.450
R-factor	0.253
Rwork	0.253
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.800	2.470
High resolution limit [Å]	2.400	2.400
Number of reflections	108311
<I/σ(I)>	13.7
Completeness [%]	97.6	98
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	100 mM NaCitrate pH 6.8, 1.29-1.59 M NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K