2H27
Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-25 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.009, 68.709, 61.133 |
| Unit cell angles | 90.00, 101.25, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 19011 | |
| Completeness [%] | 97.5 | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 5% v/v MPD, 0.04M magnesium chloride, 0.05M sodium-cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 6.00 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | sodium-cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | magnesium chloride | ||
| 7 | 1 | 2 | sodium-cacodylate |
