2H1R
Crystal structure of a dimethyladenosine transferase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.008, 82.222, 75.909 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.890 |
| R-factor | 0.20111 |
| Rwork | 0.198 |
| R-free | 0.24954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zq9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.459 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (V. 1.3.1) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.474 |
| Number of reflections | 55385 | |
| <I/σ(I)> | 9.8 | 1.95 |
| Completeness [%] | 98.9 | 87.4 |
| Redundancy | 5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 297 | 23.5% PEG3350, 0.2M Tri Lithium Citrate, 0.1M Hepes at pH7.1, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
