2H1G
ResA C74A/C77A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.06880 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.031, 59.522, 109.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.160 - 3.100 |
| R-factor | 0.238 |
| Rwork | 0.185 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1su9 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.044 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.048 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.127 | 0.296 |
| Total number of observations | 4118 | |
| Number of reflections | 5722 | |
| <I/σ(I)> | 4.6 | 2.2 |
| Completeness [%] | 96.6 | 91.9 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 20 % - 27 % PEG 4,000, 0.1 M MES pH 5.6, 0.1 M Ammonium Acetate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
