2H19
Crystal Structure of ResA Cys77Ala Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97620 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.196, 59.438, 110.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.000 - 2.000 |
| R-factor | 0.18384 |
| Rwork | 0.181 |
| R-free | 0.23682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1su9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 21619 | |
| <I/σ(I)> | 13.6 | 4 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 5.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 27 - 30 % PEG 4,000, 0.1M Sodium Citrate pH 5-5.8, 0.2 M Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
