2H18

Structure of human ADP-ribosylation factor-like 10B (ARL10B)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-12-14
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	39.406, 66.460, 39.646
Unit cell angles	90.00, 117.54, 90.00

Refinement procedure

Resolution	30.000 - 1.902
Rwork	0.201
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2al7
RMSD bond length	0.016
RMSD bond angle	1.375
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.047	0.026	0.274
Number of reflections	13666	1444	1317
<I/σ(I)>	14.1
Completeness [%]	95.4
Redundancy	3.7	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20% PEG3350, 0.2M potassium chloride, 5mM TRIS, pH 7.5, vapor diffusion, sitting drop, temperature 291K