2H17

Structure of human ADP-ribosylation factor-like 5 (ARL5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97895
Spacegroup name	P 21 21 21
Unit cell lengths	39.047, 48.772, 85.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.720 - 1.700
Rwork	0.186
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z6y
RMSD bond length	0.017
RMSD bond angle	1.469
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.720	30.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.055	0.028	0.475
Number of reflections	18505	2000	1777
<I/σ(I)>	11.7
Completeness [%]	98.9
Redundancy	6.9	6.5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	2.0M sodium formate, 0.1M BTP, pH 7.0, vapor diffusion, sitting drop, temperature 291K