2H16

Structure of human ADP-ribosylation factor-like 5 (ARL5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	94.624, 94.624, 214.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.204
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zj6
RMSD bond length	0.015
RMSD bond angle	1.384
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.053	0.026	0.266
Number of reflections	62818	7132	3410
<I/σ(I)>	15.7
Completeness [%]	93.7
Redundancy	11.1	13	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	20% PEG3350, 0.2M diammomium citrate, 5mM Tris, pH 7.5, vapor diffusion, sitting drop, temperature 291K