2H0Z
Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme bound to glucose-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 180.877, 39.809, 70.226 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
Rwork | 0.206 |
R-free | 0.23900 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.210 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 14171 | |
Completeness [%] | 96.6 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 295 | grown from: 12% PEG 4000, 1M LiCl, 100mM MES-NaOH pH 5.4, 5mM MgCl2. stabilized in: 24% PEG 4000, 1.5M LiCl, 30mM MgCl2, 100mM MES-NaOH pH 5.5, 20mM glucose-6-phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
10 | 1 | 3 | LiCl | ||
11 | 1 | 3 | MES-NaOH | ||
12 | 1 | 3 | MgCl2 | ||
13 | 1 | 3 | glucose-6-phosphate | ||
2 | 1 | 1 | LiCl | ||
3 | 1 | 1 | MES-NaOH | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 2 | PEG 4000 | ||
6 | 1 | 2 | LiCl | ||
7 | 1 | 2 | MES-NaOH | ||
8 | 1 | 2 | MgCl2 | ||
9 | 1 | 3 | PEG 4000 |