2H0Q

Crystal Structure of the PGM domain of the Suppressor of T-Cell receptor (Sts-1)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-17
Detector	ADSC QUANTUM 210
Wavelength(s)	0.967
Spacegroup name	C 1 2 1
Unit cell lengths	116.229, 74.340, 100.106
Unit cell angles	90.00, 101.46, 90.00

Refinement procedure

Resolution	98.060 - 1.820
R-factor	0.19808
Rwork	0.196
R-free	0.23599
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.357
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.060	1.860
High resolution limit [Å]	1.820	1.820
Rmerge	0.057	0.572
Number of reflections	72288
<I/σ(I)>	26	2.5
Completeness [%]	96.3	94.4
Redundancy	5.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	13% PEG4000, 0.3 M magnesium acetate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	13% PEG4000, 0.3 M magnesium acetate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	13% PEG4000, 0.3 M magnesium acetate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K