2H0I
Crystal Structure of DsbG V216M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9724 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.121, 57.201, 84.281 |
Unit cell angles | 90.00, 96.75, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
Rwork | 0.220 |
R-free | 0.25800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1v57 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | MX (SYSTEM) |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.065 | 0.255 |
Number of reflections | 21710 | |
<I/σ(I)> | 20 | 5 |
Completeness [%] | 95.9 | 76.8 |
Redundancy | 3.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.75 | 298 | 18% PEG 4000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 3.75, VAPOR DIFFUSION, SITTING DROP, temperature 298K |