2H0F
Crystal Structure of PucM in the presence of 8-azaxanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-16 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 0.97144 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 73.109, 73.109, 144.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.28100 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
| Rmerge | 0.131 | 0.046 | |
| Number of reflections | 12724 | ||
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 13.5 | 11.7 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 295 | 0.1M sodium acetate, 0.5M NaCl, 0.5M ammonium sulfate, pH 4.2, VAPOR DIFFUSION, temperature 295K |
