2H0E
Crystal Structure of PucM in the absence of substrate
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-12 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 0.97948, 0.97964, 0.97180 |
| Spacegroup name | P 42 2 2 |
| 格子定数 [Å] | 73.170, 73.170, 145.020 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 分解能 | 50.000 - 2.200 |
| R因子 | 0.224 |
| Rwork | 0.224 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.09) |
| Refinement software | CNS |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.280 |
| 分解能 [Å] (高) | 2.200 | 4.740 | 2.200 |
| Rmerge_l_obs | 0.084 | 0.053 | 0.506 |
| 独立反射数 | 20441 | ||
| <I/σ(I)> | 12.8 | ||
| 完全性 [%] | 98.2 | 99.7 | 86.5 |
| 冗長性 | 12.8 | 12.7 | 7.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 4.2 | 295 | pH 4.2, VAPOR DIFFUSION, temperature 295K |
| 1 | VAPOR DIFFUSION | 4.2 | 295 | pH 4.2, VAPOR DIFFUSION, temperature 295K |
| 1 | VAPOR DIFFUSION | 4.2 | 295 | pH 4.2, VAPOR DIFFUSION, temperature 295K |
