2GZX

Crystal Structure of the TatD deoxyribonuclease MW0446 from Staphylococcus aureus. Northeast Structural Genomics Consortium Target ZR237.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97913
Spacegroup name	P 1 21 1
Unit cell lengths	46.678, 77.324, 76.129
Unit cell angles	90.00, 98.01, 90.00

Refinement procedure

Resolution	26.790 - 2.200
R-factor	0.258
Rwork	0.258
R-free	0.28600
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.600
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.790	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.102	0.548
Number of reflections	53477
Completeness [%]	99.8
Redundancy	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	25% PEG 3350, 0.2 Ammonium acetate, 5 mM magnesium chloride, 0.1 M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K