2GZX
Crystal Structure of the TatD deoxyribonuclease MW0446 from Staphylococcus aureus. Northeast Structural Genomics Consortium Target ZR237.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.678, 77.324, 76.129 |
| Unit cell angles | 90.00, 98.01, 90.00 |
Refinement procedure
| Resolution | 26.790 - 2.200 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.790 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.102 | 0.548 |
| Number of reflections | 53477 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 25% PEG 3350, 0.2 Ammonium acetate, 5 mM magnesium chloride, 0.1 M TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
