2GZV
The cystal structure of the PDZ domain of human PICK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97925 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.588, 37.257, 29.442 |
| Unit cell angles | 90.00, 95.75, 90.00 |
Refinement procedure
| Resolution | 29.300 - 1.120 |
| R-factor | 0.15007 |
| Rwork | 0.149 |
| R-free | 0.16865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fne |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.332 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 1.220 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.057 | 0.013 |
| Number of reflections | 32802 | |
| <I/σ(I)> | 7.97 | |
| Completeness [%] | 98.4 | 94.8 |
| Redundancy | 3.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M Ammonium-acetate, 0.1 M Bis-Tris, 20% PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
