2GZR
Enterobactin and Salmochelin Hydrolase IroE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-02-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.220, 36.980, 44.208 |
| Unit cell angles | 111.47, 89.69, 102.99 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.203 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gzs |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 18258 | |
| Completeness [%] | 93.6 | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 6-10% PEG 3350, 100 mM Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
