2GZJ

Crystal Structure of the E9 DNase Domain with a Mutant Immunity Protein IM9 (D51A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.87
Spacegroup name	P 1 21 1
Unit cell lengths	44.903, 88.248, 52.235
Unit cell angles	90.00, 91.92, 90.00

Refinement procedure

Resolution	19.860 - 1.600
R-factor	0.187
Rwork	0.185
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1emv
RMSD bond length	0.012
RMSD bond angle	1.229
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.027	0.025	0.045
Number of reflections	51260
<I/σ(I)>	34.4
Completeness [%]	96.3	98.9	84.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	277	24% (W/V) PEG 4K, 100mM SODIUM ACETATE BUFFER pH 5.3, temperature 277K, VAPOR DIFFUSION, SITTING DROP