2GZ4
1.5 A Crystal Structure of a Protein of Unknown Function ATU1052 from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.987, 108.650, 80.955 |
| Unit cell angles | 90.00, 93.74, 90.00 |
Refinement procedure
| Resolution | 24.130 - 1.500 |
| R-factor | 0.15368 |
| Rwork | 0.152 |
| R-free | 0.18343 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.211 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.850 | 1.538 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.051 | 0.231 |
| Number of reflections | 119242 | |
| <I/σ(I)> | 17.07 | 2.1 |
| Completeness [%] | 96.9 | 71.07 |
| Redundancy | 2.3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M NH4 Formate, 12% PEG3350, 4%MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
