2GZ2
Structure of Aspartate Semialdehyde Dehydrogenase (ASADH) from Streptococcus pneumoniae complexed with 2',5'-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.389, 100.214, 64.370 |
| Unit cell angles | 90.00, 100.47, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.24900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.198 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (9.2SSI) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.850 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.131 | 0.353 |
| Total number of observations | 12633 | |
| Number of reflections | 43521 | |
| <I/σ(I)> | 6 | 2.2 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 2.99 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 50 mM Citrate pH 6.0 200 mM ammonium acetate 20% PEG 8000 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
