2GYW

Crystal Structure of Mus musculus Acetylcholinesterase in Complex with Obidoxime

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-5
Synchrotron site	MAX II
Beamline	I911-5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-02-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.07962
Spacegroup name	P 21 21 21
Unit cell lengths	79.060, 111.530, 227.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 2.400
R-factor	0.197
Rwork	0.196
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j06
RMSD bond length	0.012
RMSD bond angle	1.444
Data scaling software	XDS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.088	0.554
Number of reflections	79454
<I/σ(I)>	16.8	4.4
Completeness [%]	99.9	100
Redundancy	7.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	278	26-30 % PEG750MME, 0.1 M HEPES, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 278K