2GYV
Crystal structure of Mus musculus Acetylcholinesterase in complex with Ortho-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.131 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.730, 108.580, 220.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.500 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j06 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.545 |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.086 | 0.507 |
| Number of reflections | 64151 | |
| <I/σ(I)> | 19.6 | 4.8 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 26-30 % PEG750MME, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
