2GYP
Diabetes mellitus due to a frustrated Schellman motif in HNF-1a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9480 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 29.180, 42.950, 42.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | coordinates from 1JB6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.220 |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 10079 |
| <I/σ(I)> | 6 |
| Completeness [%] | 92.0 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 2 mM peptide in 100 mM Bicine buffer. Reservoir solution contained 5 mM Magnesium Formate and 30% PEG400, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
