2GXF
X-Ray Crystal Structure of Protein YybH from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR506.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97904, 0.97943, 0.96801 |
| Spacegroup name | P 41 |
| Unit cell lengths | 88.251, 88.251, 82.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.420 - 3.100 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.28600 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.420 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.112 | 0.585 |
| Number of reflections | 22660 | |
| <I/σ(I)> | 15.17 | 2.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 291 | 100mM MES (pH 6.15), 22% PEG5kMME, 200mM ammonium sulfate, and 5mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 291K |
