2GWJ

SpvB ADP-ribosylated actin: hexagonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Collection date	2004-10-19
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	96.361, 96.361, 97.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.330 - 1.900
R-factor	0.153
Rwork	0.152
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.869
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.000	99.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.051	0.025	0.728
Number of reflections	57844
<I/σ(I)>	14.4
Completeness [%]	100.0	99.7	100
Redundancy	7.3	10.8	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1