2GWA
Crystal Structure of a Complex Formed Between the DNA Holliday Junction and a Bis-Acridine Molecule.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.806 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.224, 25.142, 37.541 |
| Unit cell angles | 90.00, 110.97, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.750 |
| R-factor | 0.2769 |
| Rwork | 0.268 |
| R-free | 0.31010 |
| Starting model (for MR) | 1nqs |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.018 |
| Data reduction software | MOSFLM |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.042 | 0.093 |
| Number of reflections | 4045 | |
| <I/σ(I)> | 13.1 | 5.2 |
| Completeness [%] | 86.4 | 86.4 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 40mM SODIUM CACODYLATE PH 7.0, 12mM SPERMINE, 10% MPD, WITH 35% MPD RESERVOIR., VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | SODIUM CACODYLATE | ||
| 6 | 1 | 2 | MPD |
