2GW5
Crystal Structure of LIR-2 (ILT4) at 1.8 : differences from LIR-1 (ILT2) in regions implicated in the binding of the Cytomegalovirus class I MHC homolog UL18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.530, 62.530, 106.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.080 - 1.800 |
| R-factor | 0.2423 |
| Rwork | 0.240 |
| R-free | 0.27864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g0x |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.162 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 19241 | |
| Completeness [%] | 99.3 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 0.1M Hepes pH 7.5, 10% Isopropanol, 20% PEG 4000 streak seeded., VAPOR DIFFUSION, HANGING DROP |
