2GW4
Crystal structure of stony coral fluorescent protein Kaede, red form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-20 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.197, 81.169, 53.512 |
| Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| Rwork | 0.198 |
| R-free | 0.21300 |
| Structure solution method | MIR |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 68143 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2M Li2SO4, 2mM NiCl2, 0.1M Tris, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
