2GVM
Crystal structure of hydrophobin HFBI with detergent
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.84230 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.900, 121.600, 121.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fz6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.590 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.077 | 0.318 |
| Number of reflections | 28109 | |
| <I/σ(I)> | 20.9 | 4.52 |
| Completeness [%] | 92.0 | 91.7 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M zinc acetate, 0.1 HEPES (pH 7) LDAO-detergent as an additive, concentration in the drop 2 mM, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
