2GVG
Crystal Structure of human NMPRTase and its complex with NMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 253.070, 101.367, 148.200 |
| Unit cell angles | 90.00, 125.48, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 142302 |
| Completeness [%] | 93.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 295 | 50mM phosphate buffer ph 9.2 24% PEG 3350, 200mM NaCl BaCl2 as additive, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
