2GV2

MDM2 in complex with an 8-mer p53 peptide analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.660, 44.100, 59.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.000 - 1.800
R-factor	0.195
Rwork	0.192
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t4f
RMSD bond length	0.006
RMSD bond angle	1.600
Data scaling software	HKL-2000
Phasing software	CNX (2002)
Refinement software	CNX (2005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	9416
<I/σ(I)>	14.8	2.2
Completeness [%]	88.7	67.8
Redundancy	4.4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	2 M (NH4)2SO4, 100 mM Bicine, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K