2GUY
Orthorhombic crystal structure (space group P21212) of Aspergillus niger alpha-amylase at 1.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91835 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.799, 63.228, 74.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.590 |
| R-factor | 0.16527 |
| Rwork | 0.164 |
| R-free | 0.19623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7taa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.483 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.079 | 0.556 |
| Number of reflections | 65130 | |
| Completeness [%] | 98.6 | 90.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 296 | 30% PEG 8000, 0.2M Na-acetate, 0.1M Na-cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
