2GUW
Crystal structure of AMP Nucleosidase from Salmonella typhimurium LT2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 116.493, 170.284, 204.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.070 - 2.640 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.730 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.074 | 0.538 |
Number of reflections | 59111 | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 98.6 | 98.4 |
Redundancy | 6.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20-30% PEG MME550, 0.05M MgCl2, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |