2GUS
Conformational Transition between Four- and Five-stranded Phenylalanine Zippers Determined by a Local Packing Interaction
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793, 0.9796, 0.9681 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 37.002, 37.002, 80.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.850 - 1.750 |
| R-factor | 0.24303 |
| Rwork | 0.238 |
| R-free | 0.28702 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.421 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.850 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.404 |
| Number of reflections | 6157 | |
| <I/σ(I)> | 17 | 4.4 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 7.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris-HCl, 10mM nickel chloride, 14% PEG MME2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
