2GU6
E. coli methionine aminopeptidase in complex with NleP, 1: 2, di-metalated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-30 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.704, 64.034, 76.347 |
Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
Resolution | 13.596 - 1.700 |
R-factor | 0.209 |
Rwork | 0.198 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xnz |
RMSD bond length | 0.005 |
RMSD bond angle | 1.308 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 13.600 | 1.790 |
High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
Rmerge | 0.046 | 0.040 | 0.160 |
Total number of observations | 5694 | 25501 | |
Number of reflections | 46683 | ||
<I/σ(I)> | 11.7 | 11.8 | 4.7 |
Completeness [%] | 92.4 | 92 | 88.7 |
Redundancy | 3.9 | 3.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 15% PEG 20000, 0.1 M MES (pH 6.5) , VAPOR DIFFUSION, HANGING DROP, temperature 291K |