2GU4
E. coli methionine aminopeptidase in complex with NleP, 1: 0.5, di-metalated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.704, 64.034, 76.347 |
| Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
| Resolution | 14.853 - 1.800 |
| R-factor | 0.206 |
| Rwork | 0.195 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xnz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.284 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 14.980 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.065 | 0.031 | 0.292 |
| Total number of observations | 4797 | 21436 | |
| Number of reflections | 42210 | ||
| <I/σ(I)> | 10.6 | 12.5 | 2.6 |
| Completeness [%] | 99.7 | 94.5 | 99.6 |
| Redundancy | 3.6 | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 15% PEG 20000, 0.1 M MES (pH 6.5) , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
