2GTU

LIGAND-FREE HUMAN GLUTATHIONE S-TRANSFERASE M2-2 (E.C.2.5.1.18), MONOCLINIC CRYSTAL FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	289
Detector technology	AREA DETECTOR
Collection date	1997-03
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	53.909, 81.479, 55.621
Unit cell angles	90.00, 109.26, 90.00

Refinement procedure

Resolution	10.000 - 2.550
R-factor	0.203
Rwork	0.203
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hna
RMSD bond length	0.011
RMSD bond angle	22.507 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	3.000
High resolution limit [Å]	2.550	2.550
Rmerge	0.051	0.097
Number of reflections	12321
<I/σ(I)>	13.5	4.2
Completeness [%]	84.2	48
Redundancy	1.9	1.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Patskovska, L.N., (1998) Acta Crystallogr.,Sect.D, 54, 458. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-31 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH7.5
3	1	drop	dithiothreitol	0.2 (mM)