2GTT
Crystal structure of the rabies virus nucleoprotein-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-25 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9394 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 270.430, 281.000, 236.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.750 - 3.490 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.25520 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.346 |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.630 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Number of reflections | 226155 | |
| <I/σ(I)> | 12.15 | 3.36 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% TACSIMATE, 10% L-CYSTEIN, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% TACSIMATE, 10% L-CYSTEIN, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% TACSIMATE, 10% L-CYSTEIN, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 60% TACSIMATE | ||
| 2 | 1 | 1 | 10% L-CYSTEIN | ||
| 3 | 1 | 1 | water | ||
| 4 | 1 | 2 | 60% TACSIMATE |
