2GTR
Human chromodomain Y-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.207, 132.739, 100.361 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 39.370 - 1.900 |
| R-factor | 0.19139 |
| Rwork | 0.189 |
| R-free | 0.23332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fbm |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.668 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.370 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 73681 | |
| Completeness [%] | 96.0 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | 12% Isopropanol, 0.2M NaCitrate, 0.1M NaCacodylate pH 5.0., VAPOR DIFFUSION, HANGING DROP, temperature 300K |
