2GTC
Crystal structure of the hypthetical protein from Bacillus cereus (ATCC 14579). Northeast structural genomics Target BcR11
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793, 0.97952, 0.96800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.065, 54.609, 96.354 |
| Unit cell angles | 90.00, 118.23, 90.00 |
Refinement procedure
| Resolution | 28.300 - 2.300 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.29400 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.200 | 2.300 |
| Rmerge | 0.092 | 0.274 |
| Number of reflections | 27643 | |
| <I/σ(I)> | 30.7 | 22.4 |
| Completeness [%] | 1.0 | |
| Redundancy | 7.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 100mM Na3Citrate, 10% PEG 20k, 100 mM NH4Cl, 5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
