2GSZ

Structure of A. aeolicus PilT with 6 monomers per asymmetric unit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-02
Detector	MAR CCD 165 mm
Wavelength(s)	0.97896
Spacegroup name	C 1 2 1
Unit cell lengths	206.111, 105.244, 123.023
Unit cell angles	90.00, 111.06, 90.00

Refinement procedure

Resolution	25.000 - 4.200
R-factor	0.34634
Rwork	0.343
R-free	0.40563
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NTD of mol C and CTD of mol D from 2EWV.pdb
RMSD bond length	0.019
RMSD bond angle	1.732
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.350
High resolution limit [Å]	4.200	4.200
Number of reflections	17281
<I/σ(I)>	15.6	5.3
Completeness [%]	95.2	96.6
Redundancy	5.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	Upon set up, protein droplet contains 7.5 mg/ml protein in 9.7% PEG1000, 13.0 mM TCEP, 13.0 mM LiCl, 0.32 M AmSO4, 2.6 mM ADP, 16 mM Tris pH 7.6, 98 mM KCl, 130 mM Imidazole, 6.5% glycerol. Well contains 0.2 M AmSO4, 10% PEG 1000, unbuffered., VAPOR DIFFUSION, HANGING DROP, temperature 293K