2GSV
X-Ray Crystal Structure of Protein YvfG from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR478.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 51.048, 31.645, 53.357 |
| Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
| Resolution | 26.870 - 1.900 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.26400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.870 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | 0.353 |
| Number of reflections | 25914 | |
| <I/σ(I)> | 14.98 | 3.4 |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10mM Tris, 16% PEG3350, 180mM ammonium sulfate, 5mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
