2GSQ

GLUTATHIONE S-TRANSFERASE FROM SQUID DIGESTIVE GLAND COMPLEXED WITH S-(3-IODOBENZYL)GLUTATHIONE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-05-29
Detector	ELECTRONICS COMPUTING TECHNOLOGIES
Spacegroup name	P 31 2 1
Unit cell lengths	73.150, 73.150, 94.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.173
RMSD bond length	0.017
RMSD bond angle	0.037
Data reduction software	XENGEN
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.090
Number of reflections	15331
Completeness [%]	99.7
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-8 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	GSBzI	2 (mM)
5	1	drop	ammonium sulfate	40 (%sat)
6	1	reservoir	ammonium sulfate	60-70 (%sat)