2GSL
X-Ray Crystal Structure of Protein FN1578 from Fusobacterium nucleatum. Northeast Structural Genomics Consortium Target NR1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.304, 108.751, 80.495 |
| Unit cell angles | 90.00, 108.59, 90.00 |
Refinement procedure
| Resolution | 29.450 - 2.600 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.450 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.563 |
| Number of reflections | 60619 | |
| <I/σ(I)> | 13.31 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 100mM HEPES, 100mM Mg(NO3)2, 20% PEG8K, 5 mM DTT, Microbatch under oil, pH 7, temperature 293K |
